Root culturing of Faidherbia=Acacia albida as a source of explants for shoot regeneration
Identifieur interne : 000247 ( France/Analysis ); précédent : 000246; suivant : 000248Root culturing of Faidherbia=Acacia albida as a source of explants for shoot regeneration
Auteurs : J. Ahée [France] ; E. Duhoux [France]Source :
- Plant Cell, Tissue and Organ Culture [ 0167-6857 ] ; 1994-02-01.
Abstract
Abstract: Root culture ofFaidherbia=Acacia albida (Del.) A. Chev. can be maintained over several months by successively subculturing excised roots in modified Bonner and Devirian medium, which was found to be the best of the three media compositions tested. Adding auxins had an inhibiting effect on root growth characteristics. Mesoinositol at 0.05 mM slightly enhanced the overall elongation rate, and sucrose at 59 mM significantly increased root elongation. The effect of sucrose could not be replaced by glucose. Subcultured roots showed progressively less elongation in successive transfers. Shoots were regenerated in vitro from root segments beginning with the first passage on 1/5 strength MS medium. The large variability in the root elongation rate indicates that this technique provides an effective way to select clones with good potential for tap root growth.
Url:
DOI: 10.1007/BF00037723
Affiliations:
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ISTEX:89F5358D7F044B3406680E5712E28F0DFB1860E8Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Root culturing of Faidherbia=Acacia albida as a source of explants for shoot regeneration</title><author><name sortKey="Ahee, J" sort="Ahee, J" uniqKey="Ahee J" first="J." last="Ahée">J. Ahée</name></author><author><name sortKey="Duhoux, E" sort="Duhoux, E" uniqKey="Duhoux E" first="E." last="Duhoux">E. Duhoux</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:89F5358D7F044B3406680E5712E28F0DFB1860E8</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1007/BF00037723</idno><idno type="url">https://api.istex.fr/document/89F5358D7F044B3406680E5712E28F0DFB1860E8/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000E83</idno><idno type="wicri:Area/Istex/Curation">000E83</idno><idno type="wicri:Area/Istex/Checkpoint">000F48</idno><idno type="wicri:doubleKey">0167-6857:1994:Ahee J:root:culturing:of</idno><idno type="wicri:Area/Main/Merge">003971</idno><idno type="wicri:Area/Main/Curation">003691</idno><idno type="wicri:Area/Main/Exploration">003691</idno><idno type="wicri:Area/France/Extraction">000247</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Root culturing of Faidherbia=Acacia albida as a source of explants for shoot regeneration</title><author><name sortKey="Ahee, J" sort="Ahee, J" uniqKey="Ahee J" first="J." last="Ahée">J. Ahée</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Biotechnologie des Symbioses Forestières Tropicales (ORSTOM/CIRAD-Forêt), 45 Bis Avenue de la Belle Gabrielle, 94736, Nogent-sur-Marne</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Nogent-sur-Marne</settlement></placeName></affiliation></author><author><name sortKey="Duhoux, E" sort="Duhoux, E" uniqKey="Duhoux E" first="E." last="Duhoux">E. Duhoux</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Université Paris VII et Biotechnologie (des Symbioses Forestiéres Tropicales (ORSTOM/CIRAD-Forêt), 45 Bis Avenue de la Belle Gabrielle, 94736, Nogent-sur-Marne</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Nogent-sur-Marne</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant Cell, Tissue and Organ Culture</title><title level="j" type="sub">An International Journal on Biotechnology of Higher Plants</title><title level="j" type="abbrev">Plant Cell Tiss Organ Cult</title><idno type="ISSN">0167-6857</idno><idno type="eISSN">1573-5044</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1994-02-01">1994-02-01</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="219">219</biblScope><biblScope unit="page" to="225">225</biblScope></imprint><idno type="ISSN">0167-6857</idno></series><idno type="istex">89F5358D7F044B3406680E5712E28F0DFB1860E8</idno><idno type="DOI">10.1007/BF00037723</idno><idno type="ArticleID">BF00037723</idno><idno type="ArticleID">Art12</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0167-6857</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Root culture ofFaidherbia=Acacia albida (Del.) A. Chev. can be maintained over several months by successively subculturing excised roots in modified Bonner and Devirian medium, which was found to be the best of the three media compositions tested. Adding auxins had an inhibiting effect on root growth characteristics. Mesoinositol at 0.05 mM slightly enhanced the overall elongation rate, and sucrose at 59 mM significantly increased root elongation. The effect of sucrose could not be replaced by glucose. Subcultured roots showed progressively less elongation in successive transfers. Shoots were regenerated in vitro from root segments beginning with the first passage on 1/5 strength MS medium. The large variability in the root elongation rate indicates that this technique provides an effective way to select clones with good potential for tap root growth.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Nogent-sur-Marne</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Ahee, J" sort="Ahee, J" uniqKey="Ahee J" first="J." last="Ahée">J. Ahée</name></region><name sortKey="Duhoux, E" sort="Duhoux, E" uniqKey="Duhoux E" first="E." last="Duhoux">E. Duhoux</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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